Loading…
Electrochimical determination of uric acid, xanthine and hypoxanthine by poly(xylitol) modified glassy carbon electrode
The novel covalently modified glassy carbon electrode with poly(xylitol) was prepared using an electropolymerization technique for the simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). This new electrode presents an excellent electrocatalytic activity towards the oxi...
Saved in:
Published in: | Journal of Central South University 2014-03, Vol.21 (3), p.870-876 |
---|---|
Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | The novel covalently modified glassy carbon electrode with poly(xylitol) was prepared using an electropolymerization technique for the simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). This new electrode presents an excellent electrocatalytic activity towards the oxidation of UA, XA and HX by cyclic voltammetry (CV) method. The oxidation peaks of the three compounds were well defined and had enhanced the peak currents. The separation potentials of the oxidation peak potentials for UA-XA and XA-HX were 380 and 370 mV in CV, respectively. Using differential pulse voltammetry (DPV) method, the calibration curves in the ranges of 5–55, 1.3–75.3 and 4–59 μmol/L were obtained for HX, XA and UA, respectively. The lowest detection limits (S/N=3) were 4.5, 0.75 and 3.75 μmol/L for HX, XA and UA, respectively. The practical application of the modified electrode was demonstrated by the determination of UA, XA, HX in human urine samples. |
---|---|
ISSN: | 2095-2899 2227-5223 |
DOI: | 10.1007/s11771-014-2012-6 |