Evaluation of the effects of ozone therapy on Escherichia coli-induced cytitis in rat

• Published in: Irish journal of medical science 2013-12, Vol.182 (4), p.557-563
• Main Authors: Tasdemir, C., Tasdemir, S., Vardi, N., Ates, B., Onal, Y., Erdogan, S., Yucel, A., Aglamis, E., Yakupogullari, Y., Altıntas, R., Karaman, A.
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - pharmacology; Bacterial Translocation - drug effects; Cystitis - drug therapy; Cystitis - microbiology; Disease Models, Animal; Escherichia coli - drug effects; Escherichia coli Infections - drug therapy; Escherichia coli Infections - microbiology; Family Medicine; Female; General Practice; Glutathione Peroxidase - metabolism; Internal Medicine; Malondialdehyde - metabolism; Medicine; Medicine & Public Health; Nitric Oxide - metabolism; Original Article; Oxidative Stress - drug effects; Ozone - pharmacology; Peroxidase - metabolism; Rats; Rats, Wistar; Superoxide Dismutase - metabolism; Urinary Bladder - drug effects; Urinary Bladder - microbiology; Urinary Tract Infections - drug therapy; Urinary Tract Infections - microbiology
• ISSN: 0021-1265; 1863-4362
• DOI: 10.1007/s11845-013-0926-x

Objectives The aim of the study was to investigate the effect of ozone on oxidative/nitrosative stress and bladder injury caused by Escherichia coli in rat bladder. Methods Twenty-one Wistar-Albino-type female rats included in the study were divided into three groups of equal number: (1) sham operation (control), (2) E. coli -only (EC), (3) EC + ozone. After ozone therapy for 3 days, urine and tissue samples were obtained for biochemical, microbiological, and histopathological analysis. Results Tissue malondialdehyde (MDA), myeloperoxidase (MPO), and nitric oxide (NO) level were increased, whereas superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity was decreased in the EC group. MDA, MPO, and NO levels were decreased, whereas SOD, GPx activity was increased in the ozone-treated group. Also, there was no bacterial translocation in this group. Conclusion The results of the present study suggest that ozone may be used as an agent to protect the bladder from oxidative/nitrosative stress occurring in cystitis.