Purification of recombinant GFP produced by Agrobacterium-mediated transient expression in Nicotiana excelsior

Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium -mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification sc...

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Bibliographic Details
Published in:Cytology and genetics 2008-04, Vol.42 (2), p.86-89
Main Authors: Sindarovska, Y. R., Sheludko, Y. V., Gerasymenko, I. M., Bannikova, M. A., Kuchuk, N. V.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Human Genetics
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Summary:Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium -mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and includes ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in the obtaining of a fraction with about 85% GFP homogeneity and the protein yield of about 75%.
ISSN:0095-4527
1934-9440
DOI:10.1007/s11956-008-2003-3