PTH-C1: a rat continuous cell line expressing the parathyroid phenotype

The lack of a continuous cell line of epithelial parathyroid cells able to produce parathyroid hormone (PTH) has hampered the studies on in vitro evaluation of the mechanisms involved in the control of parathyroid cell function and proliferation. The PT-r cell line was first established from rat par...

Full description

Saved in:
Bibliographic Details
Published in:Endocrine 2014-09, Vol.47 (1), p.90-99
Main Authors: Fabbri, Sergio, Ciuffi, Simone, Nardone, Valeria, Gomes, Ana Rita, Mavilia, Carmelo, Zonefrati, Roberto, Galli, Gianna, Luzi, Ettore, Tanini, Annalisa, Brandi, Maria Luisa
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Cell Line
Cell Proliferation - drug effects
Cells, Cultured
Diabetes
Endocrine Methods and Techniques
Endocrinology
Gene Expression - drug effects
Humanities and Social Sciences
Humans
Internal Medicine
Medicine
Medicine & Public Health
multidisciplinary
Parathyroid Glands - cytology
Parathyroid Glands - metabolism
Parathyroid Hormone - genetics
Parathyroid Hormone - metabolism
Phenotype
Rats
Receptors, Calcium-Sensing - genetics
Receptors, Calcium-Sensing - metabolism
Science
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The lack of a continuous cell line of epithelial parathyroid cells able to produce parathyroid hormone (PTH) has hampered the studies on in vitro evaluation of the mechanisms involved in the control of parathyroid cell function and proliferation. The PT-r cell line was first established from rat parathyroid tissue in 1987, but these cells were known to express the parathyroid hormone-related peptide ( Pthrp ) gene, but not the Pth gene. In an attempt to subclone the PT-r cell line, a rat parathyroid cell strain was isolated and named PTH-C1. During 3 years, in culture, PTH-C1 cells maintained an epithelioid morphology, displaying a diploid chromosome number, a doubling time around 15 h during the exponential phase of growth, and parathyroid functional features. PTH-C1 cell line produces PTH and expresses the calcium sensing receptor ( Casr ) gene and other genes known to be involved in parathyroid function. Most importantly, the PTH-C1 cells also exhibit an in vitro secretory response to calcium. Altogether these findings indicate the uniqueness of the PTH-C1 cell line as an in vitro model for cellular and molecular studies on parathyroid physiopathology.
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-014-0229-7