Diagnostic Performance of rep-PCR as a Rapid Subtyping Method for Listeria monocytogenes

Previously pulsed field gel electrophoresis-typed Listeria monocytogenes isolates ( N  = 95) were tested by repetitive element sequence-based PCRs (rep-PRCs). A combined rep-PCR typing approach showed 95 % repeatability, 0.98 discriminatory power, 95.5 % sensitivity, 75 % specificity, 91.2 % predict...

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Bibliographic Details
Published in:Food analytical methods 2013-06, Vol.6 (3), p.868-871
Main Authors: Nucera, Daniele M., Lomonaco, Sara, Costa, Annalisa, Morra, Patrizia, Grassi, Maria Ausilia
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Food Science
Microbiology
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Summary:Previously pulsed field gel electrophoresis-typed Listeria monocytogenes isolates ( N  = 95) were tested by repetitive element sequence-based PCRs (rep-PRCs). A combined rep-PCR typing approach showed 95 % repeatability, 0.98 discriminatory power, 95.5 % sensitivity, 75 % specificity, 91.2 % predictive positive value, and 85.7 % predictive negative value. Hence, rep-PCR represents an efficient and rapid subtyping method for L. monocytogenes .
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-012-9496-1