Multivariate Optimisation and Validation of a Method for the Separation of Five Artificial Sweeteners by UPLC-DAD in Nine Food Matrices

A new, fast and efficient method was developed for the separation and simultaneous quantification of acesulfame-K, aspartame, cyclamate, neotame and saccharin in food by ultra-performance liquid chromatography (UPLC) and diode array detector (DAD). Univariate strategies were applied for the optimisa...

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Bibliographic Details
Published in:Food analytical methods 2015-08, Vol.8 (7), p.1824-1835
Main Authors: Dias, Cintia Botelho, Meinhart, Adriana Dillenburg, Pane, Daniela Queiroz, Ballus, Cristiano Augusto, Godoy, Helena Teixeira
Format: Article
Language:English
Subjects:
acesulfame potassium
algorithms
Analytical Chemistry
aspartame
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
food matrix
Food Science
juices
Microbiology
nectar
rapid methods
saccharin
soft drinks
solvents
tea
temperature
tomato sauce
ultra-performance liquid chromatography
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Summary:A new, fast and efficient method was developed for the separation and simultaneous quantification of acesulfame-K, aspartame, cyclamate, neotame and saccharin in food by ultra-performance liquid chromatography (UPLC) and diode array detector (DAD). Univariate strategies were applied for the optimisation of mobile phase pH, proportion of solvents in the mobile phase, flow rate and column temperature. Multivariate techniques were used for the simultaneous optimisation of 13 responses applying the Derringer and Suich desirability function. Many of the models presented lack of fit. However, it was possible to optimise the method using strategies for the restriction of the region to be investigated by the algorithm used in the desirability function. The optimised method was validated and applied to nine food matrices (soft drink, nectar, juice, ready-to-drink tea, jam, barbecue sauce, tomato sauce, instant juice and instant pudding), presenting good resolution, rapid analysis (11 min) and low use of reagents. This indicates that the UPLC is an excellent alternative for the simultaneous analysis of artificial sweeteners in foods.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-014-0056-8