Development of a Method for the Analysis of Sterols in Sterol-Enriched Deli-Style Turkey with GC-FID

Plant sterols have been recognised by the European Food Safety Authority for their cholesterol-lowering properties and are currently added to several food formulations. The objective of this study was to develop a method based on formation of sterol trimethylsilyl ether derivatives and separation an...

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Published in:Food analytical methods 2016-03, Vol.9 (3), p.724-728
Main Authors: Grasso, S, Brunton, N. P, Monahan, F. J, Harrison, S. M
Format: Article
Language:English
Subjects:
Analytical Chemistry
beta-sitosterol
campesterol
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
cholesteremic effect
Food and Drug Administration
food safety
Food Science
gas chromatography
guidelines
meat
Microbiology
stigmasterol
turkeys
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description Plant sterols have been recognised by the European Food Safety Authority for their cholesterol-lowering properties and are currently added to several food formulations. The objective of this study was to develop a method based on formation of sterol trimethylsilyl ether derivatives and separation and quantification by GC for the determination of three phytosterols (β-sitosterol, stigmasterol and campesterol) in sterol-enriched deli-style turkey. The assay was linear (concentration range 4.3–172.1 μg/ml, R ² ≥ 0.9868) and accuracy and precision were within the acceptance criteria of the U.S. Food and Drug Administration (USFDA) guidelines for method validation, set at
doi_str_mv 10.1007/s12161-015-0242-3
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Accuracy measured by relative response factor (RRF) also met the USFDA validation criteria. No matrix effects were observed. The response factors (RFs) of the three sterols differed significantly to that of the internal standard (ISTD) used, leading to RRF dissimilar to 1 (campesterol = 1.0167, stigmasterol = 1.4458, β-sitosterol = 0.9029). 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The assay was linear (concentration range 4.3–172.1 μg/ml, R ² ≥ 0.9868) and accuracy and precision were within the acceptance criteria of the U.S. Food and Drug Administration (USFDA) guidelines for method validation, set at &lt;20 % RSD at the lower limit of quantification (LLOQ) and &lt;15 % RSD for all other standards. Accuracy measured by relative response factor (RRF) also met the USFDA validation criteria. No matrix effects were observed. The response factors (RFs) of the three sterols differed significantly to that of the internal standard (ISTD) used, leading to RRF dissimilar to 1 (campesterol = 1.0167, stigmasterol = 1.4458, β-sitosterol = 0.9029). 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subjects Analytical Chemistry
beta-sitosterol
campesterol
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
cholesteremic effect
Food and Drug Administration
food safety
Food Science
gas chromatography
guidelines
meat
Microbiology
stigmasterol
turkeys
title Development of a Method for the Analysis of Sterols in Sterol-Enriched Deli-Style Turkey with GC-FID
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