A Highly Sensitive and Selective Spectrofluorimetric Method for the Determination of Nitrite in Food Products

This paper describes a simple, sensitive, and selective spectrofluorimetric method to determine trace amount of nitrite in food products using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM DA) as a fluorescent probe. The reaction of DAF-FM DA with nitrite in acidic medium resulte...

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Bibliographic Details
Published in:Food analytical methods 2016-05, Vol.9 (5), p.1293-1300
Main Authors: Wang, Qiuhua, Huang, Haiwei, Ning, Baoming, Li, Minfeng, He, Lan
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Food Science
Microbiology
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Summary:This paper describes a simple, sensitive, and selective spectrofluorimetric method to determine trace amount of nitrite in food products using 4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM DA) as a fluorescent probe. The reaction of DAF-FM DA with nitrite in acidic medium resulted in triazolofluorescein (DAF-FM T), a highly fluorescent reagent in neutral medium. The fluorescence enhancement was proportional to nitrite concentration in the range of 5.0 × 10 −8 to 1.5 × 10 −6  mol L −1 with a detection limit of 3.3 × 10 −8  mol L −1 (S/ N  = 3). The proposed method has been applied to the determination of nitrite in real food samples, with relative standard deviation (RSD) ( n  = 6) less than 5.1 % and recoveries in the range of 86.4 ~ 102.9 %.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-015-0306-4