Dispersive Micro-solid-Phase Extraction of Acaricides from Fruit Juice and Functional Food Using Cucurbituril as Sorbent

• Published in: Food analytical methods 2022-05, Vol.15 (5), p.1356-1367
• Main Authors: Gu, Yu-Xin, Yan, Tian-Ci, Yue, Zi-Xuan, Li, Min-Hui, Zheng, Hui, Wang, Shu-Ling, Cao, Jun
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Food Science; Microbiology
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-021-02209-8

A novel dispersive micro-solid-phase extraction (DMSPE) method is established for the determination of seven acaricides (clofentezine, benzomate, fenpyroximate, diafenthiuron, pyridaben, doramectin, and ivermectin) in fruit juice and functional food by ultra-high-performance liquid chromatography. In this method, these analytes were extracted from apple juice and curcuma wenyujin using cucurbituril used as the adsorbent, water as the extraction solvent, and methanol as the elution solvent. The primary parameters affecting the extraction efficiency were optimized by experiments for concentration of adsorbent, oscillation time, desorption solvent, and pH value and by response surface methodology. Under the optimal conditions, all target analytes had a good linear relationship, with correlation coefficients ranging from 0.9900 to 0.9940, the limits of detection were 0.42–5.16 ng/mL, the limits of quantification were 1.39–17.18 ng/mL, and the spiked recoveries of seven target compounds in real samples ranged from 70.04 to 94.95%. The intra-day precision RSD variations ranged from 0.13 to 0.70%, and the inter-day precision RSDs were between 0.38 and 1.4%, which had good reproducibility and repeatability. In general, DMSPE is a convenient, efficient, and environmentally friendly method for sample preparation, which has the potential for microextraction.