The Cloning and Expression of Renalase and the Preparation of Its Monoclonal Antibody

To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express...

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Bibliographic Details
Published in:Shanghai jiao tong da xue xue bao 2009-06, Vol.14 (3), p.376-379
Main Author: 王锋 汪年松 邢涛 曹阳 向海燕
Format: Article
Language:English
Subjects:
Architecture
Computer Science
DNA片段
Electrical Engineering
Engineering
Life Sciences
Materials Science
克隆与表达
单克隆抗体
基因扩增
特异性引物
重组蛋白
阳性克隆
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Summary:To obtain the recombination protein of renalase and prepare the monoclonal antibody, the human renalase gene was amplified from human kidney by specific primer and cloned the DNA fragments into the pET22b. After verification of the positive clones, the gene was transformed to E. coli BL21 to express the protein with 6His on C terminal. The Balb/c mouse was immunized with the purified protein to prepare the monoclonal antibody by hybidoma technique. The renalase protein was reconstructed and 2 strains of the hybidoma which can stable secrete renalase were obtained. The monoclonal antibody can both react with the both recombinant and human serum renalase.
ISSN:1007-1172
1995-8188
DOI:10.1007/s12204-009-0376-9