Amplification and Characterization of Bull Semen Infected Naturally with Foot-and-mouth Disease Virus Type Asia1 by RT-PCR

To investigate the security of semen biologically, 15 bull semen samples were collected （of which 5 exhibited clinical signs of Foot-and-mouth disease） and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease...

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Bibliographic Details
Published in:Virologica Sinica 2008, Vol.23 (5), p.378-382
Main Authors: Shao, Jun-jun, Chang, Hui-yun, Lin, Tong, Cong, Guo-zheng, Du, Jun-zheng, Guo, Jian-hong, Bao, Hui-fang, Shang, You-jun, Yang, Ya-min, Liu, Xiang-tao, Liu, Zai-xin, Liu, Ji-xing
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Medical Microbiology
Microbial Genetics and Genomics
Microbiology
Oncology
Virology
临床分析
分析方法
口足病
治疗方法
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Summary:To investigate the security of semen biologically, 15 bull semen samples were collected （of which 5 exhibited clinical signs of Foot-and-mouth disease） and identified by RT-PCR and virus isolation. The results indicated that the semen of the infected bulls were contaminated by Foot-and-mouth disease virus （FMDV）, but FMDV was not detected in semen samples from those bulls not showing clinical signs of Foot-and-mouth disease （FMD）. This is the first report of the presence of FMDV in bull semen due to natural infection in China. The analysis of the partial sequence of the VP1 gene showed that the virus strain isolated from semen has 97.9% identity with the virus isolated from vesicular liquid of infected bulls showing typical signs of FMD and belonged to the same gene sub-group
ISSN:1674-0769
1995-820X
DOI:10.1007/s12250-008-2980-5