Certification of Three Reference Materials for α- and γ-Tocopherol in Edible Oils

• Published in: MĀPAN : journal of Metrology Society of India 2014, Vol.29 (3), p.183-194
• Main Authors: Shehata, Adel B., Rizk, Mahmoud S., Farag, Ahmad M., Tahoun, Ibrahim F.
• Format: Article
• Language: English
• Subjects: Mathematical and Computational Physics; Mathematical Methods in Physics; Measurement Science and Instrumentation; Numerical and Computational Physics; Original Paper; Physics; Physics and Astronomy; Simulation; Theoretical
• ISSN: 0970-3950; 0974-9853
• DOI: 10.1007/s12647-014-0097-x

Tocopherols are important vegetable oil constituents, and their reliable quantitative analysis depends largely on the existence and quality of certified reference materials (CRMs) which provides traceability of the measurement results to the SI units. Since there is a lack of suitable CRMs in case of tocopherols analysis, three matrix reference materials from corn, peanut and soybean oils were certified for the content of α and γ-tocopherols. Homogeneity and stability of the prepared reference materials were studied and certification was done by two independent chromatographic analytical methods. The measurement results were statistically treated and the certified values of α and γ-tocopherol in corn, peanut and soybean oils were assigned and their associated expanded uncertainties were estimated as: 322.04 ± 8.62, 771.48 ± 15.31, 106.23 ± 5.19, 108.94 ± 9.72, 140.66 ± 5.93, 418.00 ± 14.36 mg/kg, respectively. These values were found useful for many food testing laboratories in validation of analytical methods and analytical quality control.