Comparative functional analysis of two wheat Na+/H + antiporter SOS1 promoters in Arabidopsis thaliana under various stress conditions

• Published in: Journal of applied genetics 2015-02, Vol.56 (1), p.15-26
• Main Authors: Feki, Kaouthar, Brini, Faiçal, Ben Amar, Siwar, Saibi, Walid, Masmoudi, Khaled
• Format: Article
• Language: English
• Subjects: abscisic acid; alleles; Animal Genetics and Genomics; Arabidopsis thaliana; beta-glucuronidase; Biomedical and Life Sciences; Human Genetics; Life Sciences; Microbial Genetics and Genomics; Plant Genetics and Genomics; Plant Genetics • Original Paper; promoter regions; salt stress; seedlings; sequence analysis; sodium-hydrogen antiporter; transgenic plants; wheat
• ISSN: 1234-1983; 2190-3883
• DOI: 10.1007/s13353-014-0228-7

The bread wheat TaSOS1 has been previously shown to be induced by salt stress treatment. To further investigate the regulation of the TaSOS1 gene, the two genomic fragments Pr SOS₁₋AB and Pr SOS₁₋D have been isolated and sequenced. Pr SOS₁₋AB and Pr SOS₁₋D are the promoter regions of SOS1 alleles, which are localised on genomes A and/or B, and on genome D, respectively. Sequence analysis of these two promoters revealed the presence of cis-regulatory elements which could be required for abiotic stress and abscisic acid (ABA) responsiveness. Histochemical assays of stably transformed Arabidopsis T3 plants showed that Pr SOS₁₋AB and Pr SOS₁₋D are active in this heterologous system, and their activities were almost the same at early developmental stages (4-, 8- and 12-day-old transgenic Arabidopsis seedlings). Nevertheless, β-glucuronidase (GUS) activity was detected only in plants carrying the Pr SOS₁₋AB –gusA construct grown for 20 or 30 days. Furthermore, in these plants, the application of abiotic stress produced an accumulation in gusA transcripts. Taken together, these results show that, in this heterologous dicot system and under normal growth conditions, Pr SOS₁₋AB and Pr SOS₁₋D are age-dependent and organ-specific promoters. However, in the presence of different stress conditions, the activities of these two promoters became different and only Pr SOS₁₋AB is an abiotic stress-inducible promoter at different developmental stages. Thus, Pr SOS₁₋AB can be used for the development of abiotic stress-tolerant transgenic plants.