Optimization of Critical Medium Components for Protein Production by Nostoc ellipsosporum Using Response Surface Methodology

Nostoc ellipsosporum is a pharmaceutically important cyanobacteria known for the production of anti-HIV drug called cyanovirin. A five-level four factor central composite design of response surface methodology (RSM) was performed to identify the optimal level for maximum protein production. Four med...

Full description

Saved in:
Bibliographic Details
Published in:Arabian Journal for Science and Engineering 2015-07, Vol.40 (7), p.1875-1880
Main Authors: Mittal, Deepika, Srivatsava, Anshuman, Govindasamy, Sharmila, Chandrasekaran, Muthukumaran
Format: Article
Language:English
Subjects:
Engineering
Humanities and Social Sciences
multidisciplinary
Research Article - Biological Sciences
Science
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nostoc ellipsosporum is a pharmaceutically important cyanobacteria known for the production of anti-HIV drug called cyanovirin. A five-level four factor central composite design of response surface methodology (RSM) was performed to identify the optimal level for maximum protein production. Four medium variables, PHA extract, glucose, Fe-EDTA and micronutrients, were chosen for RSM optimization study. Experimental data were analyzed by regression, and model equation was constructed. Maximum protein production was expected at the predicted optimal level of PHA extract, 2.50 % (v/v), glucose, 0.05 % (w/v), Fe-EDTA, 0.125 % (v/v) and micronutrients, 0.125 % (v/v). Validation experiment results were in good agreement with the results predicted by RSM. Results of this study showed that optimization by RSM approach improves the protein production, and also PHA extract was found to be a significant medium component for enhancing the protein synthesis.
ISSN:1319-8025
2191-4281
DOI:10.1007/s13369-015-1706-x