Plant-mediated synthesis of Ag and Se dual-doped ZnO-CaO-CuO nanocomposite using Nymphaea alba L. extract: assessment of their photocatalytic and biological properties

• Published in: Biomass conversion and biorefinery 2023-10, Vol.14 (24), p.32121-32131
• Main Authors: Sabouri, Zahra, Sabouri, Mohammad, Moghaddas, Samaneh Sadat Tabrizi Hafez, Mostafapour, Asma, Samarghandian, Saeed, Darroudi, Majid
• Format: Article
• Language: English
• Subjects: Biotechnology; Energy; Original Article; Renewable and Green Energy
• ISSN: 2190-6815; 2190-6823
• DOI: 10.1007/s13399-023-04984-2

The environmentally friendly procedure was developed for the fabrication of Ag and Se dual-doped ZnO-CaO-CuO nanocomposite (Ag and Se dual-doped ZnO-CaO-CuO-NCP) using Nymphaea alba L. leaves extract as a reducing and stabilizer agent. Different techniques have been used to characterize the physicochemical properties of the nanocomposite, including FTIR, XRD, FESEM, EDX, PSA, and UV-vis. According to FTIR spectra, functional groups or phytochemicals were seen in the structure of the synthesized nanocomposite. The crystalline nature of the nanocomposite was approved by the XRD pattern. FESEM/PSA images of nanocomposite confirmed the spherical morphology and the average size of 20–35 nm. A green manner was used for the synthesis of Ag and Se dual-doped ZnO-CaO-CuO-NCP to make an efficient photocatalyst for the removal of methylene blue (MB) dye from aqueous solutions. As shown by the photodegradation experiments, 95% of the MB dye was degraded after 120 min at a constant rate of 0.0154 min −1 . In addition, the ability of the nanocomposite was studied to investigate their cytotoxicity effects for the killing of cancer Huh-7 and normal L929 cells with the MTT assay. The IC 50 amounts for cancer Huh-7 cells at times 24, 48, and 72 h were determined as 500, 241.5, and 102.8 μg/mL, respectively, and normal L929 cells have no significant cytotoxicity. As a result of our investigations, the prepared nanocomposite will likely be effective in treating organic pollutants and biomedical wastes generated by industrial processes. Graphical abstract