Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hou...

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Bibliographic Details
Published in:Toxicology and environmental health sciences 2015-12, Vol.7 (5), p.272-276
Main Authors: Park, Dong Jun, Sekhon, Simranjeet Singh, Ahn, Ji-Young, Yoon, Hobaek, Lee, Lyon, Ko, Jung Ho, Kim, Yang Hoon, Min, Jiho
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Environmental Health
Pharmacology/Toxicology
Rapid Communication
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Summary:As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two­dimensional polyacrylamide gel electrophoresis (2­DE) after exposure of HeLa cells to melanin. The results showed 12 up­ and 3­down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.
ISSN:2005-9752
2233-7784
DOI:10.1007/s13530-015-0248-7