Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hou...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology and environmental health sciences 2015-12, Vol.7 (5), p.272-276
Main Authors: Park, Dong Jun, Sekhon, Simranjeet Singh, Ahn, Ji-Young, Yoon, Hobaek, Lee, Lyon, Ko, Jung Ho, Kim, Yang Hoon, Min, Jiho
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Environmental Health
Pharmacology/Toxicology
Rapid Communication
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73
cites cdi_FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73
container_end_page 276
container_issue 5
container_start_page 272
container_title Toxicology and environmental health sciences
container_volume 7
creator Park, Dong Jun
Sekhon, Simranjeet Singh
Ahn, Ji-Young
Yoon, Hobaek
Lee, Lyon
Ko, Jung Ho
Kim, Yang Hoon
Min, Jiho
description As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two­dimensional polyacrylamide gel electrophoresis (2­DE) after exposure of HeLa cells to melanin. The results showed 12 up­ and 3­down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.
doi_str_mv 10.1007/s13530-015-0248-7
format article
fullrecord <record><control><sourceid>crossref_sprin</sourceid><recordid>TN_cdi_crossref_primary_10_1007_s13530_015_0248_7</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1007_s13530_015_0248_7</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73</originalsourceid><addsrcrecordid>eNp9kN1KAzEQhYMoWGofwLu8QDS_m-RS6k-Fgjf1OqTZiWzZJkuygn17U-q1w8AMwzmH4UPontEHRql-rEwoQQllilAuDdFXaMG5EERrI6_bTqkiVit-i1a1Hmgr2XHW2QXaPQ8xQoE0Y_iZCtQ65IQnP89QUsU54vFUc81HP-Kp5BmGdm3KXKHHc8ZHGH0aEm69ga3HAcax3qGb6McKq7-5RJ-vL7v1hmw_3t7XT1sSOBWa2L3tJPCge8-NjEEYazopexNAaxW531vbqaCZERoEUNXvewZMaG9YD1GLJWKX3FByrQWim8pw9OXkGHVnMu5CxjUy7kzGnT384qlNm76guEP-Lqm9-Y_pFwEMZwc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells</title><source>Springer Link</source><creator>Park, Dong Jun ; Sekhon, Simranjeet Singh ; Ahn, Ji-Young ; Yoon, Hobaek ; Lee, Lyon ; Ko, Jung Ho ; Kim, Yang Hoon ; Min, Jiho</creator><creatorcontrib>Park, Dong Jun ; Sekhon, Simranjeet Singh ; Ahn, Ji-Young ; Yoon, Hobaek ; Lee, Lyon ; Ko, Jung Ho ; Kim, Yang Hoon ; Min, Jiho</creatorcontrib><description>As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two­dimensional polyacrylamide gel electrophoresis (2­DE) after exposure of HeLa cells to melanin. The results showed 12 up­ and 3­down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.</description><identifier>ISSN: 2005-9752</identifier><identifier>EISSN: 2233-7784</identifier><identifier>DOI: 10.1007/s13530-015-0248-7</identifier><language>eng</language><publisher>Seoul: Korean Society of Environmental Risk Assessment and Health Science</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Environmental Health ; Pharmacology/Toxicology ; Rapid Communication</subject><ispartof>Toxicology and environmental health sciences, 2015-12, Vol.7 (5), p.272-276</ispartof><rights>Korean Society of Environmental Risk Assessment and Health Science and Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73</citedby><cites>FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Park, Dong Jun</creatorcontrib><creatorcontrib>Sekhon, Simranjeet Singh</creatorcontrib><creatorcontrib>Ahn, Ji-Young</creatorcontrib><creatorcontrib>Yoon, Hobaek</creatorcontrib><creatorcontrib>Lee, Lyon</creatorcontrib><creatorcontrib>Ko, Jung Ho</creatorcontrib><creatorcontrib>Kim, Yang Hoon</creatorcontrib><creatorcontrib>Min, Jiho</creatorcontrib><title>Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells</title><title>Toxicology and environmental health sciences</title><addtitle>Toxicol. Environ. Health Sci</addtitle><description>As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two­dimensional polyacrylamide gel electrophoresis (2­DE) after exposure of HeLa cells to melanin. The results showed 12 up­ and 3­down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Environmental Health</subject><subject>Pharmacology/Toxicology</subject><subject>Rapid Communication</subject><issn>2005-9752</issn><issn>2233-7784</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kN1KAzEQhYMoWGofwLu8QDS_m-RS6k-Fgjf1OqTZiWzZJkuygn17U-q1w8AMwzmH4UPontEHRql-rEwoQQllilAuDdFXaMG5EERrI6_bTqkiVit-i1a1Hmgr2XHW2QXaPQ8xQoE0Y_iZCtQ65IQnP89QUsU54vFUc81HP-Kp5BmGdm3KXKHHc8ZHGH0aEm69ga3HAcax3qGb6McKq7-5RJ-vL7v1hmw_3t7XT1sSOBWa2L3tJPCge8-NjEEYazopexNAaxW531vbqaCZERoEUNXvewZMaG9YD1GLJWKX3FByrQWim8pw9OXkGHVnMu5CxjUy7kzGnT384qlNm76guEP-Lqm9-Y_pFwEMZwc</recordid><startdate>20151201</startdate><enddate>20151201</enddate><creator>Park, Dong Jun</creator><creator>Sekhon, Simranjeet Singh</creator><creator>Ahn, Ji-Young</creator><creator>Yoon, Hobaek</creator><creator>Lee, Lyon</creator><creator>Ko, Jung Ho</creator><creator>Kim, Yang Hoon</creator><creator>Min, Jiho</creator><general>Korean Society of Environmental Risk Assessment and Health Science</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20151201</creationdate><title>Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells</title><author>Park, Dong Jun ; Sekhon, Simranjeet Singh ; Ahn, Ji-Young ; Yoon, Hobaek ; Lee, Lyon ; Ko, Jung Ho ; Kim, Yang Hoon ; Min, Jiho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Environmental Health</topic><topic>Pharmacology/Toxicology</topic><topic>Rapid Communication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Dong Jun</creatorcontrib><creatorcontrib>Sekhon, Simranjeet Singh</creatorcontrib><creatorcontrib>Ahn, Ji-Young</creatorcontrib><creatorcontrib>Yoon, Hobaek</creatorcontrib><creatorcontrib>Lee, Lyon</creatorcontrib><creatorcontrib>Ko, Jung Ho</creatorcontrib><creatorcontrib>Kim, Yang Hoon</creatorcontrib><creatorcontrib>Min, Jiho</creatorcontrib><collection>CrossRef</collection><jtitle>Toxicology and environmental health sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Dong Jun</au><au>Sekhon, Simranjeet Singh</au><au>Ahn, Ji-Young</au><au>Yoon, Hobaek</au><au>Lee, Lyon</au><au>Ko, Jung Ho</au><au>Kim, Yang Hoon</au><au>Min, Jiho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells</atitle><jtitle>Toxicology and environmental health sciences</jtitle><stitle>Toxicol. Environ. Health Sci</stitle><date>2015-12-01</date><risdate>2015</risdate><volume>7</volume><issue>5</issue><spage>272</spage><epage>276</epage><pages>272-276</pages><issn>2005-9752</issn><eissn>2233-7784</eissn><abstract>As a high quantity of melanin can lead to hyperpig­mentation or skin cancer in humans and it can also activate lysosomal enzymes so in the present study the changes in lysosomal enzymes upon exposure of HeLa cells to melanin has been studied. The HeLa cells were exposed to 100 ppm melanin for 24 hours and lysosomal enzymes were extracted. The lysosomal enzymes were identified through two­dimensional polyacrylamide gel electrophoresis (2­DE) after exposure of HeLa cells to melanin. The results showed 12 up­ and 3­down regulation spots in relation to melanin exposure. It has been observed that lysosomal proteins are related to melanin to decrease the color and quantity through cellular activation of lysosomes.</abstract><cop>Seoul</cop><pub>Korean Society of Environmental Risk Assessment and Health Science</pub><doi>10.1007/s13530-015-0248-7</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 2005-9752
ispartof Toxicology and environmental health sciences, 2015-12, Vol.7 (5), p.272-276
issn 2005-9752
2233-7784
language eng
recordid cdi_crossref_primary_10_1007_s13530_015_0248_7
source Springer Link
subjects Biomedical and Life Sciences
Biomedicine
Environmental Health
Pharmacology/Toxicology
Rapid Communication
title Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T16%3A05%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-crossref_sprin&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Different%20expression%20patterns%20of%20lysosomal%20proteins%20exposed%20to%20melanin%20in%20HeLa%20cells&rft.jtitle=Toxicology%20and%20environmental%20health%20sciences&rft.au=Park,%20Dong%20Jun&rft.date=2015-12-01&rft.volume=7&rft.issue=5&rft.spage=272&rft.epage=276&rft.pages=272-276&rft.issn=2005-9752&rft.eissn=2233-7784&rft_id=info:doi/10.1007/s13530-015-0248-7&rft_dat=%3Ccrossref_sprin%3E10_1007_s13530_015_0248_7%3C/crossref_sprin%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2037-9b964e2c7da284fc3898644d8ce775f2ab9965c71837e3e05dbd1e137a81def73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true