Diesel degradation in soil catalyzed by the addition of a bioagent

The supernatant harvested from a mesophilic, molasses-fed, non-methanogenic bioreactor, which is rich in nitrogen, phosphorous, metals, free amino acids, Sporolactobacillus sp., Prevotella sp., and Clostridium sp., is diluted with tap water and tested as the bioagent to catalyze diesel degradation i...

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Bibliographic Details
Published in:International journal of environmental science and technology (Tehran) 2016-02, Vol.13 (2), p.551-560
Main Authors: Lin, Y, Lay, J.-J, Shieh, W. K
Format: Article
Language:English
Subjects:
Aquatic Pollution
Bioagent
Clostridium sp
Diesel degradation
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Science and Engineering
Original Paper
Prevotella sp
Soil
Soil Science & Conservation
Sporolactobacillus sp
Waste Water Technology
Water Management
Water Pollution Control
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Summary:The supernatant harvested from a mesophilic, molasses-fed, non-methanogenic bioreactor, which is rich in nitrogen, phosphorous, metals, free amino acids, Sporolactobacillus sp., Prevotella sp., and Clostridium sp., is diluted with tap water and tested as the bioagent to catalyze diesel degradation in soil. Outdoor experiments are performed under the following conditions to assess the effectiveness of the bioagent: diesel doses: 9-15 mg TPH/g soil; bioagent concentrations and dose: 1-5% at 60 ml/day; soil sample size: 600 g; ambient temperatures: 28-32 °C, and relative humidity: 40-82 % (TPH: total petroleum hydrocarbons). Diesel degradation in soil treated with 3 % bioagent, which proceeds at the rates of 1.04-1.55 mg TPH/g soil-day, is completed in about a week with up to 83 % efficiencies. In contrast, diesel degradation in soil sprinkled with water (60 ml/day) proceeds at the rate of 0.3 mg TPH/g soil-day that achieves 15-22 % degradation efficiencies. The addition of 3 % bioagent yields desired soil moisture content (10-15 %), soil pH (6.8-8.2), and nutrition inputs. Both Sporolactobacillus sp. and Prevotella sp. grown on molasses are robust, tolerant high diesel doses, able to utilize hydrophobic hydrocarbons, and readily adaptable to the soil environment. Most notably, prior acclimatization is not required to enable these properties.
ISSN:1735-1472
1735-2630
DOI:10.1007/s13762-015-0889-8