Isolation and expression analysis of defensin gene and its promoter from Brassica juncea

The full-length pathogen-inducible plant defensin gene encoding 80 amino acid sequences of pathogen-related gene PR12 (Bjdefensin) was isolated from Brassica juncea. Conserved domain (CD) search reveals its similarity with gamma thionin and knottins families of plant antimicrobial peptides. The prot...

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Bibliographic Details
Published in:Journal of plant diseases and protection (2006) 2017-12, Vol.124 (6), p.591-600
Main Authors: Rawat, Sandhya, Ali, Sajad, Nayankantha, N. N. Chamil, Chandrashekar, N., Mittra, Bhabatosh, Grover, Anita
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Life Sciences
Original Article
Plant Pathology
Plant Physiology
Plant Sciences
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Summary:The full-length pathogen-inducible plant defensin gene encoding 80 amino acid sequences of pathogen-related gene PR12 (Bjdefensin) was isolated from Brassica juncea. Conserved domain (CD) search reveals its similarity with gamma thionin and knottins families of plant antimicrobial peptides. The protein blast reveals more than 80% of its similarity with defensin-like protein of Brassica rapa and Camelina sativa. Gene expression studies revealed that the transcript levels of Bjdefensin gene increases significantly upon Alternaria infection, jasmonic acid and wounding treatments but was not induced by salicylic acid. To further study the stress-inducible regulation of Bjdefensin gene, its promoter (2.5 kb) was isolated and cloned upstream of GUS gene in pORER2 vector. In silico studies of Bjdefensin promoter showed many important conserved cis-elements, responsive to biotic and abiotic stresses. Histochemical GUS assay showed pathogen-inducible expression of Bjdefensin promoter after fungal infection. Bjdefensin promoter was also induced by Jasmonic acid and wounding.
ISSN:1861-3829
1861-3837
DOI:10.1007/s41348-017-0103-y