Marker assisted introgression of a major bacterial blight resistance gene, Xa38 into a rice maintainer line, APMS 6B

• Published in: Indian phytopathology 2019-03, Vol.72 (1), p.35-41
• Main Authors: Yugander, A., Sundaram, R. M., Singh, K., Prasad, M. S., Hari Prasad, A. S., Madhav, M. S., Laha, Gouri Sankar
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Life Sciences; Plant Pathology; Research Article
• ISSN: 0367-973X; 2248-9800
• DOI: 10.1007/s42360-018-00111-8

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae is a major production constraint for rice in India. We introgressed a major dominant BB resistance gene, Xa38 in the genetic background of APMS 6B, a maintainer line of APMS 6A (female parent of DRRH 3) through marker-assisted backcross breeding (MABB). The BB resistance gene, Xa38 was transferred to APMS 6B from the donor, PR 114 (Xa38) through two backcrosses. A single selected true BC 2 F 1 plant having maximum recovery of recurrent parent genome and also high degree of BB resistance was selfed to generate BC 2 F 2 s. At BC 2 F 2 , plants homozygous for Xa38 were identified and were further confirmed for the absence of two major fertility restorer genes, Rf3 and Rf4 . Eight best BC 2 F 2 plants, homozygous positive for Xa38 and homozygous negative for Rf3 and Rf4 were selected and advanced up to BC 2 F 6 . At BC 2 F 6 , these introgression lines exhibited high degree of BB resistance against multiple Xoo strains and were similar to APMS 6B in respect to different agro-morphological traits.