Transmission efficiency protocol of two ilarviruses (ApMV and ApNMV) associated with mosaic disease of apple (Malus domestica) through cleft grafting and T-budding

• Published in: Indian phytopathology 2024, Vol.77 (2), p.433-439
• Main Authors: Nabi, Sajad Un, Baranwal, Virendra K., Mir, Javid Iqbal, Raja, Wasim H., Yasmin, Salwee, Shah, Mohammad Abas, Sharma, Om Chand, Verma, Mahendra K., Parveen, Shugufta
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Life Sciences; Plant Pathology; Research Article
• ISSN: 0367-973X; 2248-9800
• DOI: 10.1007/s42360-024-00739-9

The present study of transmission of apple necrotic mosaic virus (ApNMV) in mixed infection with apple mosaic virus (ApMV) associated with mosaic disease of apple was conducted through cleft grafting and T-budding. Eight and three rootstocks were used for grafting and budding respectively with scion wood from ApMV and ApNMV infected apple cultivar Golden Delicious. Development of mosaic symptoms was scored over time and symptoms were first seen on two rootstocks MM106 and MM111 after 60 and 280 days after grafting and budding respectively. The comparative transmission efficiency of mosaic associated viruses was found more in grafted plants (61.5%) than budded plants (25%) on three rootstocks studied in both approaches. Both the viruses (ApMV and ApNMV) were confirmed from grafted/budded plants through conventional RT-PCR using specific primers. The established protocol will help in maintaining the virus isolates for long term as well as help in screening the germplasm for resistance.