Inter simple sequence repeat (ISSR) markers reveal DNA stability in pineapple plantlets after shoot tip cryopreservation

• Published in: Vegetos - International journal of plant research 2022-06, Vol.35 (2), p.360-366
• Main Authors: Villalobos-Olivera, Ariel, Ferreira, Claudia Fortes, Yanes-Paz, Ermis, Lorente, Gustavo Y., Souza, Fernanda Vidigal, Engelmann, Florent, Martínez-Montero, Marcos Edel, Lorenzo, José Carlos
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Life Sciences; Plant Anatomy/Development; Plant Breeding/Biotechnology; Plant Physiology
• ISSN: 2229-4473; 2229-4473
• DOI: 10.1007/s42535-021-00327-6

Although pineapple ( Ananas comosus var. comosus ) shoot tips have been cryopreserved but the possible effect of this process at the molecular level has not been studied. This communication describes the growth (plant fresh and dry weights; stem height; leaf length, width and area; and stem base diameter) and the Inter Simple Sequence Repeat (ISSR) analysis of pineapple plantlets of A. comosus MD-2; Red Spanish Florencia; and Hybrid 54 (Smooth Cayenne/Red Spanish) after 45 d of acclimatization. From each of these varieties, the acclimatized plants were obtained from: (1) conventional micropropagation (control 1); (2) from shoot tips submitted to pre-cryostorage conditioning treatments but not exposed to liquid nitrogen (LN) (treatment 2); and (3) from shoot tips exposed to cryostorage including use of LN (treatment 3). The ISSR-PCR method was used to study the genetic stability. There were no statistically significant differences between treatments for the phenotype indicators evaluated. On average, 45 day-old pineapple plants had 0.5 g fresh weight; 1.85 g dry weight; 12.2 cm stem height; 9.1 cm leaf length; 1.6 cm leaf width; 7.1 cm 2 leaf area; and 1.4 cm stem base diameter. Also, the potential effects of cryopreservation at the DNA level were not revealed with the eight ISSR markers used, as no polymorphic bands were recorded, which represents 100% genetic stability. As far as we know, this is the first publication on ISSR analysis of pineapple plantlets after cryopreservation.