Photobiomodulation Therapy Improves Spermatogenesis in Busulfan-Induced Infertile Mouse

About 50% of infertility is caused by men. This study aimed to investigate the efficiency of photobiomodulation on spermatogenesis in a busulfan-induced infertile mouse as a testicular degeneration treatment. Thirty-two adult NMRI male mice were divided into 4 groups: control, busulfan, PBMT 0.03 J/...

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Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2021-10, Vol.28 (10), p.2789-2798
Main Authors: Rezaei, Fatereh, Bayat, Mohammad, Nazarian, Hamid, Aliaghaei, Abbas, Abaszadeh, Hojjat-Allah, Naserzadeh, Parvaneh, Amini, Abdollah, Ebrahimi, Vahid, Abdi, Shabnam, Abdollahifar, Mohammad-Amin
Format: Article
Language:English
Subjects:
Alkylating Agents - toxicity
Animals
Busulfan - toxicity
Embryology
Infertility, Male - chemically induced
Infertility, Male - pathology
Infertility, Male - radiotherapy
Low-Level Light Therapy - methods
Male
Medicine
Medicine & Public Health
Mice
Obstetrics/Perinatology/Midwifery
Reproductive Biology: Original Article
Reproductive Medicine
Spermatogenesis - drug effects
Spermatogenesis - physiology
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Summary:About 50% of infertility is caused by men. This study aimed to investigate the efficiency of photobiomodulation on spermatogenesis in a busulfan-induced infertile mouse as a testicular degeneration treatment. Thirty-two adult NMRI male mice were divided into 4 groups: control, busulfan, PBMT 0.03 J/cm 2 , and laser 0.2 J/cm 2 . In the study, azoospermia was induced by busulfan as a testicular degeneration, and then, they were treated using photobiomodulation therapy at 0.03 J/cm2 and 0.2 J/cm 2 energy densities. Sperm parameters, stereological analysis, serum testosterone levels, together with SDH activity, MDA production oxidized as a marker for lipid peroxidation, glutathione (GSSG) and glutathione (GSH), mitochondrial membrane permeability (MMP), reactive oxygen species (ROS) production, and ATP production as well as TUNEL assay were assessed. Photobiomodulation therapy with 0.03 J/cm 2 energy densities group revealed a significant increase the testosterone hormone level and spermatogenic cells with the reduction of apoptotic cells and marked increase in GSH, ATP, and SDH levels and decrease the levels of MDA and ROS production in the busulfan-induced mice when compared with the control and sham groups. In conclusion, the photobiomodulation therapy (0.03 J/cm 2 energy density) may provide benefits on the spermatogenesis following busulfan injection and might be an alternative treatment to the patients with oligospermia and azoospermia in a dose-dependent manner. Graphical abstract
ISSN:1933-7191
1933-7205
DOI:10.1007/s43032-021-00557-8