Novel flow-injection analysis method for bioprocess monitoring

A technique for process analysis was developed based on flow-injection analysis (FIA) combined with diode-array spectrophotometry. The method demonstrates the advantages of coupling single-line FIA with diode-array detection, utilizing the stopped-flow and double injection techniques with a dual-wav...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 1991, Vol.249 (1), p.77-85
Main Authors: Chung, Suzanne, Wen, Xiaowen, Vilholm, Kirsten, De Bang, Michael, Christian, Gary, Ruzicka, Jaromir
Format: Article
Language:English
Subjects:
Biological and medical sciences
Bioprocess monitoring
Biotechnology
Fermentation
Flow system
Fundamental and applied biological sciences. Psychology
Glucose ammonia proteins
Methods. Procedures. Technologies
Others
Process analysis
Spectrophotometry
Various methods and equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A technique for process analysis was developed based on flow-injection analysis (FIA) combined with diode-array spectrophotometry. The method demonstrates the advantages of coupling single-line FIA with diode-array detection, utilizing the stopped-flow and double injection techniques with a dual-wavelength monitoring approach. A simple, robust FIA hardware system was designed and rigorously tested for on-line process application. A fed-batch fermentation served as the test bed for the development of this system, monitoring selected analytes of interest, viz., glucose, ammonium ion and total protein.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(91)87011-U