Chromatographic method for the rapid determination of variant forms of a plasminogen activator

Complex glycoproteins normally exist as a mixture of variant forms. In order to optimize production efficiency and provide meaningful product quality control, methods are needed to assess rapidly the distribution of the various forms of the protein in production batches. This paper describes a hydro...

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Bibliographic Details
Published in:Analytica chimica acta 1991, Vol.249 (1), p.201-207
Main Authors: Riggin, R.M, Haviland, C.J, Clodfelter, D.K, Kovach, P.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Glycoprotein variants
Hydrophobic interaction chromatography
Liquid chromatography
Methods. Procedures. Technologies
Others
Plasminogen activator
Proteins
Various methods and equipments
Citations: Items that this one cites
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Summary:Complex glycoproteins normally exist as a mixture of variant forms. In order to optimize production efficiency and provide meaningful product quality control, methods are needed to assess rapidly the distribution of the various forms of the protein in production batches. This paper describes a hydrophobic interaction chromatographic method for determining the important variant forms of a plasminogen activator currently under investigation as a therapeutic agent.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(91)87023-Z