Precolumn derivatization of amines in liquid chromatography using luminescent probes: Comparison of several reagents with luminarin 1

Luminarin 1 is a labelling reagent with quinolizino coumarin structure and a N-hydroxysuccinimide ester reactive function, which reacts with primary amines in 20 min at 50°C, and with secondary amines in 180 min at 70°C. Several amines were derivatized and separated by liquid chromatography (pentyla...

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Bibliographic Details
Published in:Analytica chimica acta 1992-09, Vol.266 (2), p.243-249
Main Authors: Kouwatli, H., Chalom, J., Tod, M., Farinotti, R., Mahuzier, G.
Format: Article
Language:English
Subjects:
Amines
Chemiluminescence
Fluorimetry
Liquid chromatography
Luminarin 1
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Summary:Luminarin 1 is a labelling reagent with quinolizino coumarin structure and a N-hydroxysuccinimide ester reactive function, which reacts with primary amines in 20 min at 50°C, and with secondary amines in 180 min at 70°C. Several amines were derivatized and separated by liquid chromatography (pentylamine, pyrrolidine, tyramide, proline). The limits of detection with fluorescence detection were between 160–300 fmol injected, the limit of chemiluminescence detected with bis(2,4,6-trichlorophenyl) oxalate (TCPO) and hydrogen peroxide (H 2O 2) were between 15–100 fmol injected. Derivatization limits with luminarin 1 were in the range 180–300 pmol of amine. Luminarin 1 was compared with different derivatizing reagents like FMOC, OPA and fluorscamine using fluorescence and chemiluminescence detection. FMOC and luminarin appeared to have shown the best performances in terms of derivatization and detection limits, respectively.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(92)85049-C