Development of a liposome immunosorbent assay for human interferon- y

A novel and sensitive liposome immunosorbent assay (LISA) for IFN- y is described. Biotinylated liposomes with encapsulated carboxyfluorescien comprise a universal label for immunoassays. Stability of the liposomes is proven to be adequate for long-term storage, without loss of encapsulated marker....

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Bibliographic Details
Published in:Analytica chimica acta 1994-03, Vol.287 (3), p.191-199
Main Authors: Rongen, H.A.H., van der Horst, H.M., Hugenholtz, G.W.K., Bult, A., van Bennekom, W.P., van der Meide, P.H.
Format: Article
Language:English
Subjects:
ELISA
Fluorimetry
Immunoassay
Interferon- y
Liposome immunosorbent assay (LISA)
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Summary:A novel and sensitive liposome immunosorbent assay (LISA) for IFN- y is described. Biotinylated liposomes with encapsulated carboxyfluorescien comprise a universal label for immunoassays. Stability of the liposomes is proven to be adequate for long-term storage, without loss of encapsulated marker. Sensitivity of the LISA of 6×10 −1 M is comparable to the best colorimetric immunoassays for IFN- y, and the linear detection range (6−2400×10 −12 M) exceeds the colorimetric range. Analysis of biological samples with the LISA showed no interference on the liposomes from potential disturbing factors in either plasma or blood cell culture supernatants.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(93)E0592-U