Retention behaviour of metal chelates in ion-pair reversed-phase liquid chromatography as a function of mobile phase composition with methanol-water and acetonitrile-water mobile phases

The retention behaviour of Cu(II), Co(III), Ni(II) and Fe(II) chelates with 2-(5-bromo-2-pyridylazo)-5-[ N-propyl- N-(3-sulphopropyl) amino]phenol (5-Br-PAPS) and 2-(5-nitro-2-pyridylazo)-5-[ N-propyl- N-(3-sulphopropyl)amino]-phenol (nitro-PAPS) in ion-pair reversed-phase liquid chromatography was...

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Bibliographic Details
Published in:Analytica chimica acta 1994-08, Vol.294 (1), p.69-74
Main Authors: Ohtsuka, Chizuko, Matsuzuwa, Kimiyo, Wada, Hiroko, Nakagawa, Genkichi
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Ion chromatography
Liquid chromatography
Metal chelates
Mobile phase composition
Other chromatographic methods
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Summary:The retention behaviour of Cu(II), Co(III), Ni(II) and Fe(II) chelates with 2-(5-bromo-2-pyridylazo)-5-[ N-propyl- N-(3-sulphopropyl) amino]phenol (5-Br-PAPS) and 2-(5-nitro-2-pyridylazo)-5-[ N-propyl- N-(3-sulphopropyl)amino]-phenol (nitro-PAPS) in ion-pair reversed-phase liquid chromatography was examined with mobile phases of methanolwater and acetonitrilewater binary systems containing 0.05 M sodium ion and 0.02 M Tris buffer (pH 7). The correlation of log k′ with the solubility parameter was different between the methanolwater acetonitrilewater systems. The resolution among chelates and reagents was complete with the acetonitrilewater system and far better than with the methanolwater system.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(94)85047-X