Enhanced chemiluminescence kinetic ELISA of dichlorprop methyl ester

A competitive enzyme-linked immunosorbent assay (ELISA) for dichlorprop methyl ester (DME) with an anti-rabbit secondary antibody conjugated to peroxidase was developed in polystyrene plates. The plate was developed with a luminol-H2O2-horseradish peroxidase-p-iodophenol mixture by using a fibre opt...

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Bibliographic Details
Published in:Analytica chimica acta 1996-03, Vol.321 (2-3), p.219-224
Main Authors: Navas Díaz, A., García Sánchez, F., Lovillo, J., González García, J.A.
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemiluminescence
Chemistry
Exact sciences and technology
Horseradish peroxidase
Immunoassay
Kinetic methods
Luminol
Miscellaneous
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Summary:A competitive enzyme-linked immunosorbent assay (ELISA) for dichlorprop methyl ester (DME) with an anti-rabbit secondary antibody conjugated to peroxidase was developed in polystyrene plates. The plate was developed with a luminol-H2O2-horseradish peroxidase-p-iodophenol mixture by using a fibre optic to collect the chemiluminescent emission. The standard curve was produced for 0.05 to 10 000 ng ml−1 DME. The minimum detectable concentration was 0.11 ng ml−1 and the relative standard deviation was 7.26% for a 50 ng ml−1 sample of (n = 10). The ELISA procedure was selective with respect to structurally similar compounds usually found in formulations of pesticides. This method was applied to DME added to wheat grain extracts with recoveries between 97.1 and 103.6% for 5–500 ng ml−1.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(95)00566-8