α-Cyclodextrin as a restricted access mobile phase for reversed-phase liquid chromatography with fluorimetric detection of phenolic compounds

A reversed-phase liquid Chromatographic (LC) method to determine the phenolic derivatives, p-hydroxybenzoic (PHB), ferulic and vanillic acids, and methyl paraben (MPB), was developed. The mobile phase used was 1 × 10 −2 M α-cyclodextrin/ acetate buffer pH 4.6 (1:1), that forms inclusion complexes wi...

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Bibliographic Details
Published in:Analytica chimica acta 1996-07, Vol.328 (1), p.73-79
Main Authors: Sánchez, F.García, Díaz, A.Navas, Lovillo, J, Feria, L.S
Format: Article
Language:English
Subjects:
Analytical chemistry
Beers
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Cyclodextrins
Exact sciences and technology
Fermented food industries
Fluorimetry
Food industries
Fundamental and applied biological sciences. Psychology
Liquid chromatography
Other chromatographic methods
Phenolic compounds
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Summary:A reversed-phase liquid Chromatographic (LC) method to determine the phenolic derivatives, p-hydroxybenzoic (PHB), ferulic and vanillic acids, and methyl paraben (MPB), was developed. The mobile phase used was 1 × 10 −2 M α-cyclodextrin/ acetate buffer pH 4.6 (1:1), that forms inclusion complexes with the analytes. The inclusion process enhances the fluorescence properties of the phenolic compounds, allowing detection limits in the 1–5 ng ml −1 levels. Formation constants were calculated from retention parameters. The method was applied to the determination of the phenolic compounds in beer by applying the method of standard additions.
ISSN:0003-2670
1873-4324
DOI:10.1016/0003-2670(96)00095-5