Membrane fluidity of Escherichia coli during heat-shock

The excimer-forming fluorophore dipyrenylpropane has been used to measure the relative fluidity of total membranes isolated from Escherichia coli grown at 30 or 45°C, or exposed to a heat-shock from 30 to 45°C for various periods of time. Parallel experiments were performed using [ 35S]methionine pu...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1995-11, Vol.1239 (2), p.195-200
Main Authors: Mejía, Ricardo, Gómez-Eichelmann, M.Carmen, Fernández, Marta S.
Format: Article
Language:English
Subjects:
E. coli
Escherichia coli - physiology
Fluorescence
Fluorescent Dyes
Heat shock
Heat-Shock Proteins - biosynthesis
Heat-Shock Response
Indicators and Reagents
Membrane Fluidity
Pyrenes
Time Factors
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Summary:The excimer-forming fluorophore dipyrenylpropane has been used to measure the relative fluidity of total membranes isolated from Escherichia coli grown at 30 or 45°C, or exposed to a heat-shock from 30 to 45°C for various periods of time. Parallel experiments were performed using [ 35S]methionine pulse-labeling of cells, to study the induction of heat-shock proteins (HSPs) at different times after the sudden change in E. coli growth-temperature from 30 to 45°C. Results suggest that upon an abrupt temperature upshift from 30 to 45°C, membrane fluidity adjustment to the steady-state level at the high temperature, takes place during the E. coli heat-shock response.
ISSN:0005-2736
0006-3002
1879-2642
DOI:10.1016/0005-2736(95)00152-S