Evaluation of root-mean-square radius of gyration as a parameter for universal calibration of polysaccharides

• Published in: Carbohydrate research 1987-02, Vol.160, p.215-225
• Main Authors: Fishman, Marshall L., Damert, William C., Phillips, John G., Barford, Robert A.
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; calibration; Fundamental and applied biological sciences. Psychology; Molecular biophysics; molecular weight; Physico-chemical properties of biomolecules; polysaccharides
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/0008-6215(87)80313-0

High-performance, size-exclusion chromatography columns were calibrated in average root-mean-square radii of gyration (μ R gz) by a combination of commercial “narrow” pullulan and “broad” dextran standards. The nonlinear calibration-curves were fitted by a computer-aided, iterative, least-squares procedure. Values of (μ R gz), obtained from a point-by-point transformation of the respective pullulan and dextran chromatograms by utilizing universal calibration, were compared with inputted μ R gz calibration values. For standards ranging in μ R gz value from 20.1 to 389 Å, the accuracy ranged from 1 to 15.3%. Furthermore, from relationships in the literature, μ R gz values were transformed to μ M w. These values of μ M w were comparable to, but generally less accurate than, μ M w values from direct, molecular-weight calibration.