Characterisation of four lactose monophosphates by application of 31P-, 13C-, and 1H-n.m.r. spectroscopy

By a combination of ion-exchange chromatography and h.p.l.c., two fractions ( A and B) have been obtained from pharmaceutical-grade lactose, each containing a mixture of lactose monophosphates. 31P-N.m.r. and 13C-n.m.r. spectroscopic analysis indicated A to contain the 6- and 6′-phosphates and B to...

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Bibliographic Details
Published in:Carbohydrate research 1988-03, Vol.174, p.23-36
Main Authors: Breg, Jan, Romijn, Dick, van Halbeek, Herman, Vliegenthart, Johannes F.G., Visser, Rink A., Haasnoot, Cornelis A.G.
Format: Article
Language:English
Subjects:
Atomic and molecular physics
Exact sciences and technology
Molecular properties and interactions with photons
Nuclear resonance and relaxation
Physics
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Summary:By a combination of ion-exchange chromatography and h.p.l.c., two fractions ( A and B) have been obtained from pharmaceutical-grade lactose, each containing a mixture of lactose monophosphates. 31P-N.m.r. and 13C-n.m.r. spectroscopic analysis indicated A to contain the 6- and 6′-phosphates and B to contain the 3′- and 4′-phosphates. Application of 2D- 13C- 1H COSY and 2D- 1H- 1H COSY afforded 1H-n.m.r. assignments for all protons in all four compounds. The observed 31P- 1H and 31C- 1H couplings are interpreted in terms of preferred orientations of the phosphate group in each compound.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(88)85078-X