Monitoring polysaccharide synthesis in Candida albicans

A method is described that measures the rate of [ 14C]-labelling of (1→6)-β- d-glucan, mannoprotein, and (1→3)/(1→6)-β- d-glucan during approximately one-tenth of the doubling time of Candida albicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the...

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Bibliographic Details
Published in:Carbohydrate research 1992-04, Vol.227, p.195-202
Main Authors: Brown, John A., Catley, Brian J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosynthesis
Candida albicans
cell walls
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mycology
polysaccharides
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Description
Summary:A method is described that measures the rate of [ 14C]-labelling of (1→6)-β- d-glucan, mannoprotein, and (1→3)/(1→6)-β- d-glucan during approximately one-tenth of the doubling time of Candida albicans. The labelling pattern can be used as a measure of cell-wall synthesis and to detect changes in the composition of newly constructed cell walls that might otherwise be masked by previously deposited envelope.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(92)85071-7