Preparation and crystallization of d-glucosamine oligosaccharides with dp 6-8

d-Glucosamine oligosaccharides of degree of polymerization 5–9 were separated from a chitosan hydrolyzate prepared with the cellulase of Trichoderma viride. Separation was accomplished by prefractionation with methanol-water and chromatography on a weak acid ion-exchanger, with elution by 0.01 N HCl...

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Bibliographic Details
Published in:Carbohydrate research 1993-02, Vol.239, p.227-237
Main Authors: Muraki, Einosuke, Yaku, Fumiko, Kojima, Hiroyuki
Format: Article
Language:English
Subjects:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
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Summary:d-Glucosamine oligosaccharides of degree of polymerization 5–9 were separated from a chitosan hydrolyzate prepared with the cellulase of Trichoderma viride. Separation was accomplished by prefractionation with methanol-water and chromatography on a weak acid ion-exchanger, with elution by 0.01 N HCl. Penta- to nona-saccharides comprised almost half of the total product. The hexa-hepta-, and octa-saccharides were crystallized from dilute HCl solutions as their hydrochloride salts. Conductometric titration to determine the glucosamine content and elemental analysis showed that this procedure is effective for the preparation of highly pure oligosaccharides.
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(93)84217-T