Structural studies of a neutral polysaccharide produced by Alcaligenes latus

The structure of a neutral polysaccharide, isolated from the culture medium of Alcaligenes latus strain B-16, has been investigated by monosaccharide analysis, methylation analysis, one-(1D) and two-dimensional (2D) 1H and 13C NMR spectroscopy, and enzymatic digestion, and by MALDI-TOF-MS and LC-MS...

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Bibliographic Details
Published in:Carbohydrate research 1996-10, Vol.293 (2), p.213-222
Main Authors: Nohata, Yasuhiro, Azuma, Jun-ichi, Kurane, Ryuichiro
Format: Article
Language:English
Subjects:
Alcaligenes latus
Mannofucopyranan
Neutral polysaccharide
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Summary:The structure of a neutral polysaccharide, isolated from the culture medium of Alcaligenes latus strain B-16, has been investigated by monosaccharide analysis, methylation analysis, one-(1D) and two-dimensional (2D) 1H and 13C NMR spectroscopy, and enzymatic digestion, and by MALDI-TOF-MS and LC-MS analyses of oligosaccharides isolated from a partial acid hydrolysate. The polysaccharide has the linear disaccharide repeating unit →2)-α- D-Manp-(1→3)-α- L-Fucp-(1→
ISSN:0008-6215
1873-426X
DOI:10.1016/0008-6215(96)00200-5