Transient effects in the nanosecond laser photolysis of carboxyhemoglobin: “CAGE” recombination annd spectral evolution of the protein

The transient light absorption changes at 400-600 nm observed following photodissociation of human adult carboxy-hemoglobin in aqueous solution (pH 7.0) by pulsed laser irradiation (7 ns fwhm, λ = 529 nm) reveal that geminate recombination is occurring during ≈ 100 ns after the dissociation. They al...

Full description

Saved in:
Bibliographic Details
Published in:Chemical physics letters 1979-06, Vol.64 (1), p.11-16
Main Authors: Alpert, B., El Mohsni, S., Lindqvist, L., Tfibel, F.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The transient light absorption changes at 400-600 nm observed following photodissociation of human adult carboxy-hemoglobin in aqueous solution (pH 7.0) by pulsed laser irradiation (7 ns fwhm, λ = 529 nm) reveal that geminate recombination is occurring during ≈ 100 ns after the dissociation. They also show that the spectrum of the photodeligated hemoglobin initially is slightly different from that of stable deoxyhemoglobin but develops into the latter in ≈5 μs. The recombination kinetics vary with the excitation condiitions (duration, intensity).
ISSN:0009-2614
1873-4448
DOI:10.1016/0009-2614(79)87265-6