Effect of transmethylation-reaction and increased levels of cAMP on superoxide generation of guinea-pig macrophages induced with wheat germ agglutinin and phorbor myristate

• Published in: FEBS letters 1983-04, Vol.154 (2), p.383-386
• Main Authors: Yagawa, Katsuro, Itoh, Tokuo, Tomoda, Akio
• Format: Article
• Language: English
• Subjects: Animals; Bucladesine - pharmacology; cAMP; Cyclic AMP - metabolism; Dinoprostone; Guinea Pigs; Lectins - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Methylation; Oxygen - metabolism; Phorbols - pharmacology; Phorbor myristate; Prostaglandins E - pharmacology; Superoxide generation; Superoxides - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Transmethylation of phospholipids; Tubercidin - pharmacology; Wheat germ agglutinin; Wheat Germ Agglutinins
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(83)80187-2

Superoxide (O 2 −) generation of guinea-pig macrophages induced by wheat germ agglutinin (WGA) was suppressed to a great extent by the inhibition of transmethylation with 3′-deazaadenosine. When macrophages were stimulated with phorbor myristate (PMA) instead of WGA, the suppression of O 2 − generation of macrophages was observed to be slight despite the presence of 3′-deazaadenosine. These results were confirmed under various conditions. Thus the WGA-stimulated O 2 − generation of macrophages is probably associated with transmethylation, but the PMA-stimulated O 2 − generation is not. WGA-stimulated O 2 − generation of macrophages was also inhibited in the presence of dibutyryl cAMP or prostaglandin E 2 (PGE 2), substances that increase intracellular cAMP, but PMA-stimulated O 2 − generation was only slightly affected by these compounds. These results suggest that the mechanism for O 2 − generation of macrophages caused by WGA is different from that for O 2 − generation caused by PMA.