Cloning and DNA sequence of the genes for two bacteriophage T5 tRNAs Ser

Bacteriophage T5 BglII/ HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAs Ser (tRNA Ser 1 and tRNA Ser 2) with anticodons UGA and GG...

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Bibliographic Details
Published in:FEBS letters 1983-07, Vol.158 (1), p.123-127
Main Authors: Kryukov, V.M., Ksenzenko, V.N., Kaliman, A.V., Bayev, A.A.
Format: Article
Language:English
Subjects:
Bacteriophage T5
DNA sequencing
Isoacceptor tRNA
RNA/DNA hydridization
tRNA gene
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Summary:Bacteriophage T5 BglII/ HindIII DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAs Ser (tRNA Ser 1 and tRNA Ser 2) with anticodons UGA and GGA, respectively. The main unusual structural feature of these tRNAs is the presence of extra non-basepaired nucleotides in the joinings of stem ‘b’ with stems ‘a’ and ‘c’.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)80690-5