Inhibition of protein synthesis by the cordycepin analog of (2′–5′)ppp(Ap) n A, (2′–5′)ppp(3′dAp) n 3′ dA, in intact mammalian cells

• Published in: FEBS letters 1983-06, Vol.157 (1), p.205-209
• Main Authors: Lee, Choongeun, Suhadolnik, Robert J.
• Format: Article
• Language: English
• Subjects: (2′–5′)A n -dependent endoribonuclease; (2′–5′)Oligoadenylate; (2′–5′)p33′dA n; (2′–5′)p3A n; (2′–5′)ppp(3′ dAp) n 3′ dA; (2′–5′)ppp(Ap) n A; Calcium phosphate co-precipitation; Cordycepin; endonuclease; Endonuclease activation; Protein synthesis inhibition
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(83)81146-6

The introduction of the cordycepin analog of (2′–5′)A n , (2′–5′)ppp(3′ dAp) n 3′ dA [referred to as (2′–5′)p33′ dA n ], into mouse L929 cells and cultured human fibroblasts resulted in a dose‐dependent inhibition of protein synthesis which was comparable to the inhibition observed by (2′–5′)ppp(Ap) n A [referred to as (2′–5′)p3A n ]. The inhibition of protein synthesis by (2′–5′)p33′ dA n was much more persistent than that of the naturally occurring (2′–5′)p3A n following prolonged incubation of cells. Furthermore, the (2′–5′)p3A n was cytotoxic to mammalian cells in culture, whereas the (2′–5′)p33′dA n was not.