Characterization of oligosaccharides from the urine of loco-intoxicated sheep

Two major oligosaccharides were isolated by preparative HPLC from the urine of a locoweed-fed sheep. Analysis by gas-liquid chromatography and mass-spectrometry indicated compositions of (Man) 4(GlcNAc) 2 and (Man) 5(GlcNAc) 2, respectively. Structures were determined by digestion with α-D-mannosida...

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Bibliographic Details
Published in:FEBS letters 1983-10, Vol.163 (1), p.104-109
Main Authors: Sadeh, Shoshana, Warren, Christopher D., Daniel, Peter F., Bugge, Birgitte, James, Lynn F., Jeanloz, Roger W.
Format: Article
Language:English
Subjects:
Acetylglucosamine - analysis
Animals
Biological and medical sciences
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, High Pressure Liquid
Female
Gas Chromatography-Mass Spectrometry
GlcNAc, 2-acetamido-2-deoxy-D-glucose
Locoweed
Man, D-mannose
Mannose - analysis
Medical sciences
Oligosaccharide
Oligosaccharides - urine
Plant Poisoning - urine
Plant Poisoning - veterinary
Plant poisons toxicology
Sheep
Sheep Diseases - urine
Structure
Toxicology
Urine
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Summary:Two major oligosaccharides were isolated by preparative HPLC from the urine of a locoweed-fed sheep. Analysis by gas-liquid chromatography and mass-spectrometry indicated compositions of (Man) 4(GlcNAc) 2 and (Man) 5(GlcNAc) 2, respectively. Structures were determined by digestion with α-D-mannosidase and endo-β- N-acetylglucosaminidases D and H, and comparison of the products by HPLC with synthetic standards, and oligosaccharides isolated from human mannosidosis urine. Incubation with an exo-β- N-acetylglucosaminidase was without effect.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(83)81173-9