Intermediates in 16-androstene biosynthesis in neonatal porcine testis

• Published in: FEBS letters 1984-02, Vol.167 (1), p.103-108
• Main Authors: Kwan, T.K., Honour, J.W., Gower, D.B.
• Format: Article
• Language: English
• Subjects: 16-Androstene; 16-Dehydropregnenolone; 16-dehydropregnenolone, 3β-hydroxy-5,16-pregnadien-20-one; 16α-hydroxypregnenolone, 3β,16α-dihydroxy-5-pregnen-20-one; 17-hydroxypregnenolone, 3β,17-dihydroxy-5-pregnen-20-one; 4-pregnene-3,20-dione; 5-androstenediols, 5-androstene-3β,17α(β)-diol; 5-pregnenediol, 5-pregnene-3β,20β-diol; 5α-androstenone, 5α-androst-16-en-3-one; an-α, 5α-androst-16-en-3α-ol; Analytical, structural and metabolic biochemistry; andien-β, 5,16-androstadien-3β-ol; Androstenes - biosynthesis; Animals; Biological and medical sciences; dehydroepiandrosterone, 3β-hydroxy-5-androsten-17-one; Fundamental and applied biological sciences. Psychology; Gas Chromatography-Mass Spectrometry; Male; Microsomes - metabolism; MO, methyl oxime; MU, methylene units; Neonate; Other biological molecules; Porcine testis; Pregnenolone; Pregnenolone - metabolism; pregnenolone, 3β-hydroxy-5-pregnen-20-one; progesterone; Ref. Cpd., reference compound; Swine; Terpenes, steroids. Hormones; Testis - metabolism; TMS, trimethylsilyl ether
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(84)80841-8

The metabolism of pregnenolone has been studied in microsomal preparations of testes from 3-week old piglets. Metabolites identified after derivatization by capillary gas-chromatography and further by combined gas chromatography-mass spectrometry were: 17-hydroxy- and 16α-hydroxypregnenolone, dehydroepiandrosterone, 5-androstenediols, 5-pregnenediol and andien-β. Pregnenolone and 16-dehydropregnenolone, previously not separable by other methods, were successfully resolved as methyl oximetrimethylsilyl ethers using capillary column gas-chromatography.