The synergistic effect of serotonin and epinephrine on the human platelet at the level of signal transduction

Simultaneous addition to platelets of submaximal amounts of excitatory agonists acts synergistically in provoking secretory and aggregatory responses. By measuring changes in intracellular free Ca 2+ concentration, inositol phospholipid metabolism and protein phosphorylation, we verified whether syn...

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Bibliographic Details
Published in:FEBS letters 1987-07, Vol.219 (2), p.283-288
Main Authors: de Chaffoy de Courcelles, D., Roevens, P., Van Belle, H., De Clerck, F.
Format: Article
Language:English
Subjects:
Aminoquinolines
Biological and medical sciences
Blood coagulation. Blood cells
Blood Platelets - drug effects
Blood Platelets - physiology
Blood Proteins - metabolism
Calcium - blood
Drug Synergism
Epinephrine
Epinephrine - pharmacology
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
Human
Humans
Inositol Phosphates - blood
Inositol phospholipid
Ketanserin
Kinetics
Molecular and cellular biology
PA, phosphatic acid
Phosphorylation
PIP2, phosphatidylinositol 4,5-bisphosphate PIP, phosphatidylinositol 4-phosphate
Platelet
Platelet Aggregation - drug effects
Serotonin
Serotonin - pharmacology
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Summary:Simultaneous addition to platelets of submaximal amounts of excitatory agonists acts synergistically in provoking secretory and aggregatory responses. By measuring changes in intracellular free Ca 2+ concentration, inositol phospholipid metabolism and protein phosphorylation, we verified whether synergism could be evidenced at the level of signal transduction. Challenging platelets with epinephrine only induced minor changes on the measured parameters. However, when added together with serotonin, epinephrine amplified mobilisation of intracellular C 2+, PA formation, PIP formation, protein kinase C and myosin light chain kinase activity as compared to the alterations induced by serotonin alone. It is concluded that synergistic effects on simultaneous addition of serotonin and epinephrine might originate at the level of signal transduction.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80236-3