Catecholamines increase nerve growth factor mRNA content in both mouse astroglial cells and fibroblast cells

Previous studies have shown that catecholamines increase the nerve growth factor (NGF) content in medium conditioned by mouse L-M fibroblast cells and mouse astroglial cells. In this study, the NGF mRNA levels in these cells were measured by Northern blot analysis. In astroglial cells treated with e...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1989-04, Vol.247 (2), p.463-467
Main Authors: Furukawa, Yoshiko, Tomioka, Noboru, Sato, Wakao, Satoyoshi, Eijiro, Hayashi, Kyozo, Furukawa, Shoei
Format: Article
Language:English
Subjects:
Albuterol - pharmacology
Animals
Astrocytes - drug effects
Astrocytes - metabolism
Biological and medical sciences
BSA, bovine serum albumin
Catecholamine
Catecholamines - pharmacology
Catechols - pharmacology
Cell physiology
CM, conditioned medium
CNS, central nervous system
DA, 3-hydroxytyramine
DMEM, Dulbecco's modified Eagle's medium
Dopamine - pharmacology
EIA, enzyme immunoassay
EN, epinephrine
Epinephrine - pharmacology
FCS, fetal calf serum
Fibroblast, Astroglial cell
Fibroblasts - drug effects
Fibroblasts - metabolism
Fundamental and applied biological sciences. Psychology
Inducer
Kinetics
Methylcatechol, 4
Mice
Molecular and cellular biology
NE, norepinephrine
Nerve growth factor
Nerve Growth Factors - genetics
Neurotransmission
NGF, nerve growth factor
Norepinephrine - pharmacology
Nucleic Acid Hybridization
Phenylephrine - pharmacology
PNS, peripheral nervous system
RNA, Messenger - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Previous studies have shown that catecholamines increase the nerve growth factor (NGF) content in medium conditioned by mouse L-M fibroblast cells and mouse astroglial cells. In this study, the NGF mRNA levels in these cells were measured by Northern blot analysis. In astroglial cells treated with epinephrine (EN), the cellular NGF mRNA level increased prior to accumulation of NGF in the culture medium. 3-Hydroxytyramine (DA) and norepinephrine (NE) also increased the cellular NGF mRNA content. An increased level of NGF mRNA elicited by EN was also observed in mouse L-M cells. These results indicate that catecholamines enhance NGF synthesis of L-M fibroblast cells and astroglial cells by increasing the cellular content of NGF mRNA. The present results also indicate that the effects of catecholamines are not mediated by adrenergic receptors.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81391-2