The psb C start codon in Synechocystis sp. PCC 6803

The translation start codon for psb C, the gene encoding CP43, a chlorophyll‐binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site‐directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psb D‐I had previously been ass...

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Bibliographic Details
Published in:FEBS letters 1990-01, Vol.260 (1), p.135-137
Main Authors: Carpenter, Shelly D., Charite, Jeroen, Eggers, Beth, Vermaas, Wim F.J.
Format: Article
Language:English
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Summary:The translation start codon for psb C, the gene encoding CP43, a chlorophyll‐binding protein of photosystem II, has been identified for the cyanobacterium Synechosystis sp. PCC 6803 using site‐directed mutagenesis. An AUG codon, about 50 bases upstream from the end of psb D‐I had previously been assumed to be the translation start site of psb C. However, the fact that the AUG codon is not present in psb C from several other organisms, whereas a GUG codon 14 bases upstream from the end of psb D‐I is strictly conserved suggests that CP43 translation starts at the latter codon. Mutation of GUG, but not of AUG, led to a loss of CP43 and photoautotrophic growth, indicating that the GUG codon is the sole initiation site for translation of the CP43 protein in Synechocystis sp. PCC 6803.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80085-W