The α 2 B adrenergic receptor of undifferentiated neuroblastoma × glioma hybrid NG108‐15 cells, interacts directly with the guanine nucleotide binding protein, G i 2

• Published in: FEBS letters 1990-09, Vol.269 (2), p.430-434
• Main Authors: McClue, Steven J., Milligan, Graeme
• Format: Article
• Language: English
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(90)81209-7

In membranes of undifferentiated neuroblastoma × glioma hybrid cell line NG108‐15, the apparent specific binding of [ 3 H]yohimbine measured in the presence of 1 μM noradrenaline, was increased substantially by the presence of the poorly hydrolysed analogue of QTP, guanylyl‐imidodiphosphate (Gpp[NH]p) or by preincubation of membranes with antibodies against the C‐terminal decapeptide of the α subunit of the G‐protein G i 2. Such an effect was not produced by antibodies against the equivalent region of G o α G i 3α or G s α or from non‐immune serum. By contrast, total specific binding of [ 3 H]yohimbine was not modified by co‐incubation with Gpp[NH]p or by preincubation with the antibodies from any of the anti‐G protein antisera. These results demonstrate a direct interaction of the α 2 B adrenergic receptor of NG108‐15 cells with G i 2.