Formylmethanofuran: Tetrahydromethanopterin formyltransferase from Methanosarcina barkeri Identification of N 5‐formyltetrahydromethanopterin as the product

Formylmethanofuran: tetrahydromethanopterin formyltransferase was purified from methanol grown Methanosarcina barkeri to apparent homogeneity and characterized with respect to its molecular and kinetic properties. The enzyme was found to be very similar to the formyltransferase from H2/CO2 grown Met...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1990-11, Vol.275 (1-2), p.226-230
Main Authors: Breitung, J., Thauer, R.K.
Format: Article
Language:English
Subjects:
1 U
1 μmol/min
Formylmethanofuran
Formyltetrahydromethanopterin
H4MPT
MES
Methanobacterium thermoautotrophicum
Methanofuran
Methanosarcina barkeri
MFR
MOPS
N-[Tris(hydroxymethyl)methyl]glycine
Tetrahydromethanopterin
Tricine
β-morpholinoethane sulfonic acid
γ-morpholinopropane sulfonic acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Formylmethanofuran: tetrahydromethanopterin formyltransferase was purified from methanol grown Methanosarcina barkeri to apparent homogeneity and characterized with respect to its molecular and kinetic properties. The enzyme was found to be very similar to the formyltransferase from H2/CO2 grown Methanobacterium thermoautotrophicum. It also catalyzed the formation of N 5‐formyltetrahydromethanopterin rather than of N 10‐formyltetrahydromethanopterin from formylmethanofuran and tetrahydromethanopterin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)81477-6