Widespread distribution of Gqα/Gllα detected immunologically by an antipeptide antiserum directed against the predicted C-terminal decapeptide

Antisera were raised to a synthetic peptide which represents the predicted C-terminal decapeptide of the α subunit of the G-proteins Gq and Gll. Competitive ELISA indicated that antiserum CQ2 displayed strong reactivity against this peptide. Antiserum CQ2 identified an apparently single polypeptide...

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Bibliographic Details
Published in:FEBS letters 1991-08, Vol.287 (1), p.171-174
Main Authors: Mitchell, Fiona M., Mullaney, lan, Godfrey, Philip P., Arkinstall, Steve J., Wakelam, Michael J.O., Milligan, Graeme
Format: Article
Language:English
Subjects:
G-protein
Inositol phosphate
Transmembrane signalling
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Summary:Antisera were raised to a synthetic peptide which represents the predicted C-terminal decapeptide of the α subunit of the G-proteins Gq and Gll. Competitive ELISA indicated that antiserum CQ2 displayed strong reactivity against this peptide. Antiserum CQ2 identified an apparently single polypeptide of 42 kDa which was expressed widely. The mobility of this polypeptide in SDS-PAGE was not modified by pretreatment of cells with pertussis toxin, indicating that it was not a substrate for this toxin. Furthermore, the levels and mobility of this polypeptide were unaltered by treatment of cells with cholera toxin, defining that it was not related to G sα.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)80043-3