Promiscuity of GABA A-receptor β3 subunits as demonstrated by their presence in α1, α2 and α3 subunit-containing receptor subpopulations

Polyclonal antibodies were raised in rabbits against the GABA A-receptor β3 subunit peptide sequence, KQSMPREGHGRHMDR-NH 2 coupled to keyhole limpet haemocyanin. These anti-β3 379–393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na + deo...

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Bibliographic Details
Published in:FEBS letters 1991-12, Vol.295 (1), p.81-83
Main Authors: Pollard, Simon, Duggan, Michael J., Stephenson, F.Anne
Format: Article
Language:English
Subjects:
Antibody
Benzodiazepine
GABA A
Immunoaffinity
Purification
Receptor
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Summary:Polyclonal antibodies were raised in rabbits against the GABA A-receptor β3 subunit peptide sequence, KQSMPREGHGRHMDR-NH 2 coupled to keyhole limpet haemocyanin. These anti-β3 379–393 antibodies immunoprecipitated in a dose-dependent manner specific benzodiazepine agonist binding sites from Na + deoxycholate extracts of bovine cerebral cortex. In immunoblots, anti-β3 379–393 antibodies recognised two species with M fr 59 900 and M fr 57 200 in all preparations tested, which included crude detergent-solubilised, benzodiazepine affinity chromatography-purified receptor, anti-α1 324–341 antibody, anti-Cys α2 414–424 antibody and anti-Cys α3 454–467 antibody immunoaffinity-purified GABA A-receptor subpopulations. These results provide evidence for the ubiquity and promiscuity of the GABA A-receptor β3 subunit.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)81390-T