Translation enhancing properties of the 5′-leader of potato virus X genomic RNA

• Published in: FEBS letters 1992-01, Vol.296 (3), p.267-270
• Main Authors: Zelenina, D.A., Kulaeva, O.I., Smirnyagina, E.V., Solovyev, A.G., Miroshnichenko, N.A., Fedorkin, O.N., Rodionova, N.P., Morozov, S.Yu, Atabekov, J.O.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; Biological and medical sciences; Cell-Free System; Cloning, Molecular; Codon; DNA, Viral; Fundamental and applied biological sciences. Psychology; Genetics; GUS; Microbiology; Molecular Sequence Data; neomycin phosphotransferase; NPT; Plant virus; Plant Viruses - genetics; potato virus X; Protein Biosynthesis; PVX; Rabbits; Recombinant DNA; Recombinant Fusion Proteins; RNA, Viral - genetics; RNA, Viral - metabolism; Solanum tuberosum - microbiology; Translational enhancement; Virology; β-glucuronidase
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80301-V

The double-stranded DNA copy corresponding to the 5′-nontranslated αβ-leader of potato virus X (PVX) genomic RNA (positions −3 to −85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and β-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the αβ-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.